Ethanol vapour-fixation of rat lung for immunocytochemistry investigations

Ethanol-vapour fixation of rat lung has been successfully employed in the i mmunocytochemical detection of the gastrin mucin antigen termed mucin 5AC w ithout the need of additional antigen retrieval steps. This procedure gives good morphological preservation and provides all the benefits associated w ith the microscopic examination of inflated lung tissue. This simple fixati on technique provides another option for use in immunocytochemical investig ations of rodent lung and could be adapted for other species. (C) 2001 Else vier Science B.V. All rights reserved.