Generic approach to chiral separations: Chiral capillary electrophoresis with ternary cyclodextrin mixtures

A generic approach to chiral separations using capillary electrophoresis is outlined. The generic method was developed by investigating the interactio ns of charged (sulfobutyl ether-beta -cyclodextrin, SBE-beta -CD) and neutr al cyclodextrins (primarily gamma- and dimethyl-beta -cyclodextrin) in a lo w pH buffer (50 mM sodium dihydrogen phosphate, pH 2.5). Using a background electrolyte (BGE) containing 5% w/w SBE-beta -CD, 25 mM gamma -cyclodextri n (gamma -CD) and 25 mM dimethyl-beta -CD (DM-P-CD) dissolved in the phosph ate buffer, resolution of more than 90% of the 26 randomly selected racemic pharmaceutical drugs or their synthetic intermediates was achieved. (C) 20 01 John Wiley & Sons, Inc.