The purpose of this study was to determine if black-tailed prairie dogs (Cy
nomys ludovicianus Ord) influence harvester ant nest density and species co
mposition within the shortgrass prairie biome of southwestern Kansas. Two t
reatments were established: areas colonized by prairie dogs and areas not c
olonized by prairie dogs. We recorded 183 harvester ant nests of 3 species,
Harvester ant nest density did not differ significantly between prairie do
g colonies (3.08 nests ha(-1)) and non-colonized shortgrass prairie sites (
4.54 nests ha(-1)), but species composition did. Pogonornyumex rugosus Emer
y was the most frequent species on prairie dog colonies where it accounted
for 87 % of ant nests present, as opposed to 33 % on sites where prairie do
gs were absent. Pogonomyrmex barbatus Smith was the most abundant species o
n non-colonized areas, making up 49 % of the ant nests sampled. Pogonomyrme
x occidentalis Cresson comprised 11 % of ant nests sampled, and was nearly
absent from prairie dog colonies (20 nests on non-colonized sites vs. 1 nes
t on prairie dog colonies). The average number of harvester ant species fou
nd per site was consistently greater on sites where prairie dogs were absen
t.