Double-blind controlled study on the effects of dietary diacylglycerol on postprandial serum and chylomicron triacylglycerol responses in healthy humans

Objective: The effects of dietary diacylglycerol (DG) on postprandial lipem ia in healthy humans were investigated. Methods: Forty normolipidemic male volunteers ingested fat emulsions contai ning either DG oil or triacylglycerol (TG) oil, at different doses: 10 g (n = 13), 20 g (n = 10) and 44 g (n = 17). Two test emulsions were given at s even-days intervals in random order. Fatty acid compositions of the test oi ls had been adjusted to be equal. Fasting and postprandial serum lipid conc entrations in each I:roup and plasma lipoprotein lipids in the 20 g-fat ing estion group were measured during the postprandial intervals. Results: When DG emulsion was ingested, serum TG concentrations were signif icantly lower (p < 0.05) in the late postprandial phase, i.e., 4 hours, 6 h ours as compared to the TG emulsion. The magnitude of postprandial lipemia (the area bounded by the curve above the fasting concentration) after inges tion of 44 g-DG emulsion was significantly less than that of 44 g-TG emulsi on (6.54 +/- 5.12 and 8.45 +/- 7.54 mmol.h/L, mean +/- SD, respectively). C hylomicron TG, cholesterol, and phospholipid concentrations at 4 hours afte r ingestion of DG emulsion were significantly lower (p < 0.05) than those a fter the ingestion of TG emulsion at the same time point. No marked differe nces were observed for VLDL, LDL and HDL lipids between the test emulsions. Conclusion: In the usual range of fat intake (10-44 g), postprandial respon se after ingestion of DO emulsion was significantly less than that after in gestion of TG emulsion in healthy human subjects.