Chromosome composition of some citrus species and cultivars based on the chromomycin A(3) (CMA) banding patterns

Chromosomes which were derived enzymatically from young leaves of the follo wing species and cultivars were sequentially stained with Giemsa, chromomyc in A(3) (CMA), and quinacrine mustard (QM): 'Suisho-Buntan', 'Banpeiyu' and 'Ban-okan' pummelo (Citrus grandis [L.] Osb.), fingered citron (C. medica L. var. sarcodactylis), 'Okitsu-Wase' satsuma mandarin (C, unshiu Marc.), k oji (C. leiocarpa hort. ex Tanaka), and 'Duncan' grapefruit (C. paradisi Ma cf.). The chromosome compositions are: 3A+3C+3D+9E in 'Suisho-Buntan' 'Ban-okan', 2B+8D+8E in fingered citron, 1A+1C+8D+8E in 'Okitsu-Wase', 1C+8D+9E in koj i and 2A+1B+1C+6D+8E in 'Duncan'. In 'Banpeiyu', the composition of the 17 chromosomes was 1A+2B+2C+4D+8E; the remaining chromosome could not be disce rned whether it was A or B. In all species and cultivars except fingered ci tron, some chromosome CMA bands are not paired, indicating that some exist as partial homologue chromosomes. Type-D and type-E chromosomes are predominant types in species or cultivar, such as fingered citron,'Okitsu- Wase', and koji. They are considered to h ave differentiated earlier during their phylogeny. Therefore, it is thought that type-D and type-E chromosomes are the basic types in citrus. 2A+1B+3C +3D+9E in citrus.