Pj. Chang et St. Liu, Function of the intercistronic region of BRLF1-BZLF1 bicistronic mRNA in translating the Zta protein of Epstein-Barr virus, J VIROLOGY, 75(3), 2001, pp. 1142-1151
Zta, a transcription factor encoded by Epstein-Barr virus, is efficiently t
ranslated from a BRLF1-BZLF1 bicistronic mRNA. In this study, we demonstrat
e that inserting a stem-loop structure, which is known to block ribosome sc
anning, in the 5' region of the intercistronic region does not prevent the
translation of a luciferase reporter protein from the bicistronic mRNA fuse
d with the firefly luciferase gene, suggesting that the translation does no
t involve translation reinitiation. Mutational analyses reveal that the reg
ion between nucleotides 86 and 125 (region I) of the intercistronic region
is essential for the translation. Meanwhile, the region between nucleotides
126 and 165 (region II) is also important since, without this region, the
translation is inefficient. The region I sequence is partially complementar
y to the sequence between nucleotides 1489 and 1524 of 18S rRNA. This homol
ogy is significant, since disrupting the homology reduces the translation e
fficiency. Furthermore, luciferase is efficiently translated if the entire
intercistronic region is replaced with a sequence complementary to the regi
on between nucleotides 1401 and 1560 of the 18S rRNA. We hypothesize that R
ta may assist 40S ribosome in recognizing the region I sequence to start a
scanning process for Zta translation.