Unusual lipid composition of a Bacillus sp isolated from Lake Pomorie in Bulgaria

The lipid composition of a Bacillus sp., isolated from Lake Pomorie in Bulg aria, was unusual and consisted of 26 different fatty acids between C-12 an d C-26, With anteiso C-15-C-17 saturated fatty acids predominating. The fur an fatty acid, 10, 13-epoxy-11-methyloctadeca-10,12-dienoic-acid, was also identified, a new finding for this genus. The hydrocarbons consisted of 30 different monounsaturated hydrocarbons, between C-25 and C-30, With the iso -iso, iso-anteiso, anteiso-anteiso, iso-normal, and anteiso-normal methyl b ranching for odd-numbered chains, and the iso-iso, iso-anteiso, iso-normal, and anteiso-normal methyl branching for even-numbered chains. The double b ond positions in these hydrocarbons were determined by dimethyl disulfide d erivatization followed by CC-MS, and the double-bond cis configuration was confirmed by infrared spectroscopy. Same previously unknown hydrocarbons in bacteria, such as (Z)-3,21-dimethyl-9-tricosene, (Z)-3,21-dimethyl-10-tric osene, (Z)-2,24-dimethyl-11-pentacosene, and (Z)-2,25-dimethyl-13-hexacosen e were identified. Sterols were detected and were based on the sitosterol n ucleus.