Some efficient methods to correct confocal images for easy interpretation

In this paper we have explained some efficient methods to collect artefacts in confocal laser beam scanning microscope (CLSM) images. The main aim is to enhance object features such that they become clearly visible for intera ctive evaluation and to reduce the overall noise so that the automatic segm entation and feature measurement can be done easily. A simple automatic-thr esholding technique, and a straightforward method to restore the light inte nsity along the depth of the image stack are proposed. Another problem asso ciated with the CLSM is the non-isotropic resolution. We have presented an interpolation technique based on XOR contouring and morphing to virtually i nsert the image slices in the image stack for improving the axial resolutio n. This interpolation technique has the merits of both contour- and intensi ty-based interpolations. Results of application of these methods on CLSM da ta are shown. (C) 2001 Elsevier Science Ltd. All rights reserved.