rSNP_Guide, a database system for analysis of transcription factor bindingto target sequences: application to SNPs and site-directed mutations

rSNP_Guide is a novel curated database system for analysis of transcription factor (TF) binding to target sequences in regulatory gene regions altered by mutations. It accumulates experimental data on naturally occurring site variants in regulatory gene regions and site-directed mutations. This data base system also contains the web tools for SNP analysis, i.e,, active appl et applying weight matrices to predict the regulatory site candidates alter ed by a mutation. The current version of the rSNP_Guide is supplemented by six sub-databases: (i) rSNP_DB, on DNA-protein interaction caused by mutati on; (ii) SYSTEM, on experimental systems; (iii) rSNP_BIB, on citations to o riginal publications; (iv) SAMPLES, on experimentally identified sequences of known regulatory sites; (v) MATRIX, on weight matrices of known TF sites ; (vi) rSNP_Report, on characteristic examples of successful rSNP_Tools imp lementation. These databases are useful for the analysis of natural SNPs an d site-directed mutations. The databases are available through the Web, htt p://wwwmgs.bionet.nsc.ru/mgs/ systems/rsnp/.