Stability of several LHRH antagonists against proteolytic enzymes and identification of degradation products by mass spectrometry

Authors
Braun, K Kuhl, P Bernd, M Kutscher, B
Citation
K. Braun et al., Stability of several LHRH antagonists against proteolytic enzymes and identification of degradation products by mass spectrometry, PHARMAZIE, 56(1), 2001, pp. 45-49
Citations number
30
Categorie Soggetti
Pharmacology & Toxicology
Journal title
PHARMAZIE
ISSN journal
00317144 → ACNP
Volume
56
Issue
1
Year of publication
2001
Pages
45 - 49
Database
ISI
SICI code
0031-7144(200101)56:1<45:SOSLAA>2.0.ZU;2-4
Abstract
In this study stabilities of several LHRH antagonists against proteolytic e nzymes are compared. For the enzymatic tests 15 proteases which differ in b oth substrate specificity and pH optimum were selected. The cyclic and two linear antagonists proved to be extraordinarily stable against the enzymes used over an incubation time of 50 h. Some degradation products were identi fied by high-performance liquid chromatography combined with mass spectrome try.