Purification and characterization of a lectin from the mushroom Mycoleptodonoides aitchisonii

Authors
Kawagishi, H Takagi, J Taira, T Murata, T Usui, T
Citation
H. Kawagishi et al., Purification and characterization of a lectin from the mushroom Mycoleptodonoides aitchisonii, PHYTOCHEM, 56(1), 2001, pp. 53-58
Citations number
14
Categorie Soggetti
Agricultural Chemistry","Animal & Plant Sciences
Journal title
PHYTOCHEMISTRY
ISSN journal
00319422 → ACNP
Volume
56
Issue
1
Year of publication
2001
Pages
53 - 58
Database
ISI
SICI code
0031-9422(200101)56:1<53:PACOAL>2.0.ZU;2-J
Abstract
A lectin was isolated from the mushroom Mycoleptodonoides aitchisonii by me ans of affinity chromatography on bovine submaxillary mucin (BSM)-Toyopearl and gel filtration on Superose 12 HR10/30 using a FPLC system. This lectin is composed of four identical 16 kDa subunits and the molecular mass of th e intact lectin was estimated to be 64 kDa by gel filtration. In a hemagglu tination inhibition assay, it exhibited strong sugar-binding specificity to wards asialo-BSM among the mono- or oligo-saccharides and glycoproteins tes ted. The binding specificity of the lectin was also examined by surface pla smon resonance analysis. (C) 2001 Elsevier Science Ltd. All rights reserved .