Large-scale production of wheat and triticale double haploids through the use of a single-anther culture method

A total of 257 parental wheat and 38 triticale lines were used for anther c ulture. On average, 2.1 green wheat haploids were obtained per spike. This response occurred irrespective of the origin of the material (Germany, Fran ce, Sweden or UK) and 5 years of testing. Triticale responded with 5.3 gree n haploids per spike. Using the criterion that one parental line should giv e at least one green haploid per spike in the screening experiment, green h aploids were produced from 88 out of 91 F-1 wheat breeding combinations and from each of 21 F-1 and F-2 triticale bleeding combinations. An average of 4.7 green plants were obtained per spike from the wheat production program me, while the triticale programme gave an average of 6.2 green plants per s pike. A single medium supplemented with different hormones for anthers and embryos was used for culture of both crops.