Down-regulation of the human CDC16 gene after exposure to ionizing radiation: A possible role in the radioadaptive response

We have used the method of differential display of mRNAs to search for chan ges in gene expression associated with radioadaptation triggered by low dos es of ionizing radiation in human lymphoblasts, We isolated a cDNA designat ed PB13 that was down-regulated as early as 1 h after irradiation with 4 Gy in cells adapted by a pre-exposure to a dose of 2 cGy, compared to 3 h in nonadapted cells (4 Gy alone). Northern analysis confirmed the differential expression of a 2.4-kb transcript that was repressed for at least 10 h aft er irradiation, The major part of the PB13 cDNA was identical to the human CDC16 mRNA, When using either PB13 or CDC16 cDNA as probes, similar radiati on-induced alterations in gene expression were observed, Expression of the CDC16 gene was also repressed after oxidative stress with H2O2. The CDC16 p rotein belongs to the anaphase-promoting complex (APC) that controls progre ssion through mitosis, The repression of expression of the CDC16 gene by io nizing radiation could result in delayed progression of damaged cells throu gh mitosis, This cycle delay would occur earlier in adapted cells and would allow a more rapid and efficient repair that could contribute to the toler ance to subsequent irradiation. (C) 2001 by Radiation Research Society.