Ma. Freulet-marriere et al., Evidence for association of lipopolysaccharide with Pseudomonas fluorescens strain MF0 porin OprF, RES MICROB, 151(10), 2000, pp. 873-876
Lipopolysaccharide (LPS) was found to be associated with the major outer me
mbrane protein OprF of the psychrotrophic bacterium Pseudomonas fluorescens
MFO, using two OprF purification procedures. OprF, purified under mild con
ditions, presented two types of association with LPS: tight (tLPS) and slig
ht (sLPS), both of type R. LPS protected OprF from heat modification and tr
ypsin degradation and facilitated the reincorporation of purified OprF into
an artificial lipid bilayer without affecting its pore-forming activity. T
he size of the OprF channel depended on cell, growth temperature, as did th
e extent of LPS phosphorylation: we suggest that LPS may be involved in mod
ifications of OprF pore formation. (C) 2000 Editions scientifiques et medic
ales Elsevier SAS.