Inhibition of the growth of human hepatoma cell line both in vitro and in vivo by transducing CKI gene p21(WAF-1) With GE7 targeting gene delivery system

The EGF receptor-mediated targeting gene delivery system GE7 was used to tr ansduce exogenous gene pCEP-p21(WAF-1) into human hepatocellular carcinoma cell both in vitro and in vivo. After in vitro transduction of the exogenou s gene, the growth of the cell lines SMMC-7721 and EEL-7402 was significant ly inhibited compared with the control. On day 8 the inhibition rates of th e above cell lines reached 56.0% and 66.7%, respectively. The in vivo exper iment showed that the growth of human hepatoma transplanted in nude mice in jected with GE7 gene delivery system subcutaneously once a week for 3 weeks was remarkably inhibited compared with that of untransfected control. The average tumor weight of the experiment group was (0.083 +/- 0.043) g, while that of the control group was (0.281 +/- 0.173) g. The difference is signi ficant (P<0.05), It was indicated that GE7 gene delivery system could effic iently transduce exogenous gene pCEP-p21(WAF-1) into hepatoma cell with hig h EGF receptor expression, and inhibit the cell growth with high efficacy b oth in vivo and in vitro.