Cultivar identification of 'Yuzu' (Citrus junos Sieb. ex Tanaka) and related acid citrus by leaf isozymes

Leaf extracts of 27 'Yuzu' and related acid citrus cultivars were analyzed using polyacrylamide gel electrophoresis for isozyme variation of glutamate oxaloacetate transaminase (GOT) and shikimate dehydrogenase (SDH). SDH yie lded 12 different isozyme phenotypes and six cultivars were discriminated b y this enzyme alone. GOT produced 10 different isozyme phenotypes and four cultivars were separated. When both enzyme systems were taken together, 16 cultivars (59%) were uniquely discriminated and the rest could be classifie d into four groups of 2-4 cultivars each. Mutation originated cultivars cou ld not be discriminated, Differences between cultivars suggested that isozy mes may provide useful markers for cultivar identification. (C) 2001 Elsevi er Science B.V. All rights reserved.