Chemiluminescence determination of some fluoroquinolone derivatives in pharmaceutical formulations and biological fluids using [Ru(bipy)(2)(+)(3)]-Ce(IV) system

A new chemiluminescence (CL) method using flow injection has been described for the rapid and sensitive determination of three fluoroquinolone derivat ives, namely ofloxacin, norfloxacin and ciprofloxacin hydrochloride. The me thod is based on the CL reaction of the studied fluoroquinolones with tris( 2,2'-bipyridyl)ruthenium(II) [Ru(bipy)(3)(2+)] and Ce(IV) in sulfuric acid medium. Under the optimum conditions, the CL intensity is proportional to t he concentration of the drugs in solution over the range 0.05-7.0 mug ml(-1 ) for norfloxacin, 0.05-6.0 mug ml(-1) for ciprofloxacin hydrochloride and 0.003-0.7 mug ml(-1) for ofloxacin. The limits of detection (s/n = 3) were 3.1 x 10(-8) M norfloxacin, 2.6 x 10-8 M ciprofloxacin hydrochloride and 5. 5 x 10(-9) M ofloxacin. The method was applied successfully to the determin ation of these compounds in dosage forms and biological fluids. (C) 2001 El sevier Science B.V. All rights reserved.