Differential gene regulation of interleukin-1 ligands and receptors in bovine peripheral blood neutrophils and mononuclear cells in response to E-coli lipopolysaccharide (LPS)

The proinflammatory cytokine, interleukin-1, plays a prominent role in the inflammatory reactions that characterize numerous diseases. In this study, we examined the gene expression for bovine IL-1 ligands and receptors by bo vine peripheral blood mononuclear cells (MNCs) and neutrophils (PMNs) in re sponse to E. coli lipopolysaccharide (LPS) in vitro. Gene expression of mRN A for IL-1 alpha, IL-1 beta, IL-1 receptor antagonist (IL-1ra), type 1 IL-1 receptor, type 2 IL-1 receptor, and IL-1 beta converting enzyme (ICE), wer e measured by a semi-quantitative RT-PCR technique. LPS had little effect o n type 1 IL-1R expression in MNC, whereas, it strongly upregulated type 1 I L-1R expression in PMNs. Co-incubation of PMNs with LPS and bovine recombin ant IL-1 beta had little additional effect on type 1 IL-1R expression. Incu bation of MNCs with LPS resulted in up-regulation of IL-1 beta, IL-1ra, and type 2 IL-1R, no change in IL-1 alpha, and a decrease in ICE gene expressi on. Incubation of PMNs with LPS up-regulated IL-1 beta gene expression, whe reas, IL-1 alpha, IL-1ra, type 2 IL-1R and ICE were unchanged. This study p rovides evidence for differential regulation of gene products of the bovine IL-1 family by peripheral blood mononuclear cells (MNC) and neutrophils (P MNs) in response to E. coli LPS. (C) 2000 Elsevier Science B.V. All rights reserved.