Molecular typing of Salmonella weltevreden and Salmonella chincol by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)

Genomic DNA of Salmonella weltevreden (10 isolates from poultry, two isolat es each from raw vegetables and river water) and S. chincol (15 isolates fr om poultry) were characterized by pulsed-field gel electrophoresis (PFGE) a nd enterobacterial repetitive intergenic consensus-polymerase chain reactio n (ERIC-PCR) analysis. These isolates originated from a single location in Kajang, Selangor. The results of the PFGE and ERIC-PCR were analysed and co mparisons were made using GelCompar software. ERIC-PCR with primers ERIC1R and ERIC2 discriminated the S. weltevreden into five clusters and two singl e isolates and S. chincol into two clusters and two single isolates at a si milarity level of 80%, respectively. PFGE produced a single cluster and eig ht single isolates for S. weltevreden, and one cluster and 11 single isolat es for S. chincol at a similarity level of 80% after digestion with the res triction enzyme XbaI, respectively. These results demonstrate that both PFG E and ERIC-PCR are suitable tools for molecular typing of the isolates exam ined.