Cyanophora paradoxa: Nucleotide sequence and phylogeny of the nucleus encoded muroplast fructose-1,6-bisphosphate aldolase

Immunoscreening of a C. paradoxa expression library against water soluble m uroplast ("cyanelle") proteins resulted in isolation of a clone encoding th e nucleus-encoded muroplast class-II fructose-1,6-bisphosphate aldolase (cl ass-II FBA(closed integral)). Its nucleotide sequence was determined. The 1 432 bp insert, derived fl om a single-copy gene transcript, bears a reading frame of 1206 bp in length, representing 402 amino acids with 346 amino ac ids of mature protein. The leading amino acids match structural features ne cessary for precursor import across chloroplast envelope membranes. In phyl ogenetic tree topology, the investigated mature FBA clusters within type B FBAs with Synechocystis sp. as nearest neighbor. This is the first report o f a Type B class-II FBA sequence of plastids.