Crystallization and preliminary crystallographic studies of an antimicrobial protein from Pharbitis nil

Authors
Ha, SC Min, D Koo, JC Kim, Y Yun, DJ Cho, MJ Kim, KK
Citation
Sc. Ha et al., Crystallization and preliminary crystallographic studies of an antimicrobial protein from Pharbitis nil, ACT CRYST D, 57, 2001, pp. 263-265
Citations number
12
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
57
Year of publication
2001
Part
2
Pages
263 - 265
Database
ISI
SICI code
0907-4449(200102)57:<263:CAPCSO>2.0.ZU;2-0
Abstract
An antimicrobial protein from seeds of Pharbitis nil (Pn-AMP) which shows a n antifungal activity towards several agriculturally important plant pathog ens has been crystallized in the presence of equimolar N-acetylglucosamine with sodium citrate as precipitant. The crystal belongs to the hexagonal sp ace group P6(1)22 (or P6(5)22), with unit-cell parameters a = b = 29.33 (5) , c = 133.44 (12) Angstrom. Native data were collected using a crystal at 1 00 K to a resolution of 1.78 Angstrom.