Preliminary structural studies of Escherichia coli isopentenyl diphosphateisomerase

Authors
Oudjama, Y Durbecq, V Sainz, G Clantin, B Tricot, C Stalon, V Villeret, V Droogmans, L
Citation
Y. Oudjama et al., Preliminary structural studies of Escherichia coli isopentenyl diphosphateisomerase, ACT CRYST D, 57, 2001, pp. 287-288
Citations number
17
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
57
Year of publication
2001
Part
2
Pages
287 - 288
Database
ISI
SICI code
0907-4449(200102)57:<287:PSSOEC>2.0.ZU;2-8
Abstract
Escherichia coli isopentenyl diphosphate isomerase, an enzyme catalyzing a key step in isoprenoid biosynthesis, has been produced in selenomethionyl f orm. The protein was purified and crystallized by the hanging-drop vapour-d iffusion method. Crystals display trigonal symmetry, with unit-cell paramet ers a = b = 71.3, c = 61.7 Angstrom, and diffract to 1.45 Angstrom resoluti on.