Crystallization and preliminary X-ray analysis of Borrelia burgdorferi outer surface protein C (OspC)

Authors
Kumaran, D Eswaramoorthy, S Dunn, JJ Swaminathan, S
Citation
D. Kumaran et al., Crystallization and preliminary X-ray analysis of Borrelia burgdorferi outer surface protein C (OspC), ACT CRYST D, 57, 2001, pp. 298-300
Citations number
30
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
57
Year of publication
2001
Part
2
Pages
298 - 300
Database
ISI
SICI code
0907-4449(200102)57:<298:CAPXAO>2.0.ZU;2-F
Abstract
Single crystals of the outer surface protein C (OspC) from Borrelia burgdor feri HB19 have been obtained by the vapor-diffusion method. These crystals belong to space group P2(1), with unit-cell parameters a = 66.218, b = 46.1 13, c = 112.079 Angstrom, beta = 99.30 degrees, and diffract to at least 2. 2 Angstrom resolution. Native data have been collected from flash-frozen cr ystals at the National Synchrotron facility of Brookhaven National Laborato ry. There are two dimers per asymmetric unit, related by a non-crystallogra phic twofold axis and a pseudo-translational symmetry.