Embolisms and refilling in the maize leaf lamina, and the role of the protoxylem lacuna

The proportion of embolized vessels in the veins of maize leaf laminas was measured during 24 h by direct counting in snap-frozen samples in the cryo- scanning electron microscope. All vessels were sap filled at night. Vessels of intermediate and small veins, and the small tracheary elements of later al veins, were sap filled throughout the 24 h. The large metaxylem Vessels of lateral veins were embolized during the day. The percentage of these ves sels embolized was maximum (>70%) at 1400, and declined during the afternoo n to 20% at dusk. Leaf water potential reached a minimum (-1.2 MPa) at dusk . The protoxylem lacuna of the lateral Veins was much less embolized than t he large vessels, although it was of comparable diameter. The observations are interpreted in terms of the refilling hypothesis that is part of the co mpensating pressure theory of water transport.