A method is described that allows, for the first time, intraoperative vibra
tion modes assessment of the acoustically stimulated stapes by means of sca
nning laser Doppler interferometry (LDI). The study was designed to answer
the following questions: 1) Is LDI practical for taking measurements during
surgery? 2) Are the results comparable to the findings in temporal bone pr
eparations? and 3) Do the vibration characteristics of the stapes change af
ter the posterior incudal ligament is detached from the incus? Seven patien
ts with profound bilateral hearing loss who were undergoing cochlear implan
tation were included in the study. The measurement system was easily applic
able for intraoperative measurements and allowed contact-free analysis with
very high accuracy. No major differences in the results from the live huma
n subjects and temporal bone preparations were observed. The stapes movemen
t was predominantly pistonlike at the lower frequencies and became complex
at higher frequencies. Sacrificing the posterior incudal ligament had no st
atistically significant effect on stapes vibration.