Germinated and nongerminated conidial suspensions for testing of susceptibilities of Aspergillus spp. to amphotericin B, itraconazole, posaconazole, ravuconazole, and voriconazole

The effect of germinated and nongerminated conidia of Aspergillus spp, on t he fungistatic (National Committee for Clinical Laboratory Standards docume nt M38-P) and fungicidal activities (MICs and minimal fungicidal concentrat ions [MFCs] respectively) of amphotericin B, itraconazole, posaconazole (SC H56592), ravuconazole (BMS-207147), and voriconazole was evaluated. MFCs we re the lowest drug dilutions that showed fewer than three colonies (99.9% k illing). Overall, the MICs (0.12 to 4 mug/ml) and MFCs (0.5 to >8 mug/ml) o f all of the agents tested with both inocula were the same or within 2 dilu tions for the 72 isolates. Therefore, MICs and MFCs can be obtained with co nvenient and standardized nongerminated conidia.