Approaches for deciphering the structural basis of low temperature enzyme activity

An increasing number of enzymes active at low temperature are being studied to help determine the structural features important for cold-activity. Thi s review examines the diversity of prokaryotic cold-active enzymes and the features proposed to account for low temperature activity. We then consider the difficulty of identifying the key structural features needed for cold- activity and the need to compare enzymes having different temperature optim a from phylogenetically related organisms to determine features responsible for low temperature activity. In addition to studying naturally occurring enzymes, directed evolution experiments are discussed as methods for examin ing the proposed mechanisms influencing the thermal dependence of activity. (C) 2000 Elsevier Science B.V. All rights reserved.