We describe collection and purification of peripheral blood CD34(+) cells f
rom volunteer, normal donors and allogeneic stem cell donors. A total of 98
aphereses were performed on 68 volunteer donors using peripheral venous ac
cess, The mean number of nucleated cells collected was 4.6 x 10(10) which i
ncluded 1,9 x 10(8) CD34(+) cells corresponding to 2.7 x 10(6) CD34(+) cell
s/kg, The number of CD34(+) cells collected did not differ between males an
d females but did correlate with the donor's weight and the total number of
nucleated cells collected. The Nexell Isolex 300i cell separator was used
to isolate CD34+ cells from 30 of the collections. A mean of 0.36% of the t
otal cells was recovered and included 43 +/- 18% of the CD34(+) cells. CD34
(+) cells represented 85 +/- 11% of the recovered cells. The total number o
f CD34(+) cells recovered was not influenced by the number of nucleated cel
ls placed on the Isolex 300i, The percentage of CD34(+) cells recovered was
not related to the number of CD34(+) cells placed on the Isolex 300i. The
purity of the final product was influenced by the number of CD34(+) cells b
ut not the total number of nucleated cells. An additional 38 CD34(+) cell i
solations were performed on normal allogeneic stem cell donors with similar
results. These observations further support the safety and feasibility of
peripheral blood CD34(+) cell collection and purification.