Characterization of chromosomal aberrations in lung cancer cell lines by cross-species color banding

Using cross-species color banding (RxFISH) and chromosome painting techniqu es, chromosomal aberrations were investigated in six lung cancer cell lines (NCI-H524, H865, H522, H1373, H358, A549). Each cell line had a variable n umber of numerical and structural cytogenetic aberrations. White NCI-H524, -H865, and -H522 had near diploidy, NCI-H358, -H1373, and A549 had near tri ploidy, The origins of the marker chromosomes were further identified by Rx FISH and chromosome painting: Nonrandom chromosomal rearrangements were see n on 1p, 3q, 5p10-p15, 6q13q-21, 7q22-q31, 9p32, 15q22-qter, 17p, 17q21-q25 , and 21. These abnormal cytogenetic findings indicate that multiple geneti c lesions are associated with the development of lung cancer, and thus, the se might be possible candidate regions for the abnormal genes involved in l ung cancer. (C) 2001 Elsevier Science Inc. All rights reserved.