Protein S-100 beta in brain and serum after deep hypothermic circulatory arrest in rabbits: Relationship to perivascular astrocytic swelling

The aim of this study was to evaluate the relationship between the kinetic patterns of the protein S-100 beta, an astroglial cell marker, and its immu nohistochemical expression in the brain in rabbits that underwent cardiopul monary bypass with deep hypothermic circulatory arrest. Fourteen New Zealand rabbits (weight, 3.1+/-0.25 kg) were anaesthetised, in tubated and mechanically ventilated. Four animals were not connected to the cardiopulmonary bypass and served as controls. Ten animals were perfused a ccording to a uniform protocol. After systemic cooling, deep hypothermic ci rculatory arrest was induced for 60 minutes. After reperfusion and rewarmin g, the animals were weaned from bypass and sacrificed. In the brain, astroc yte reactivity for S-100 beta was evaluated immunocytochemically (DPC(R) Im mustain) and the serum concentrations of S100 beta were analysed using a co mmercially available immunoluminometric kit (Byk-Sangtec(R), Dietzenbach, G ermany). In all experimental animals a significant increase of the serum concentrati on of the protein S-100 beta was found immediately after reperfusion and th e termination of cardiopulmonary bypass. In comparison with the control ani mals, increased staining of S-100 beta was found in the astroglial cells an d swollen astrocytic end-feet in the perivascular regions. There were fewer signs of neuronal call injury of neurones in the hippocampus structure. In conclusion, astrocytic activation and S-100 beta overexpression seems to precede the neurodegeneration following deep hypothermic circulatory arres t. The marked perivascular cell swelling may support the assumption of repe rfusion injury of the astroglial cell complex that forms the blood-brain ba rrier, which may be indicative of the source of the released S-100 beta int o the blood stream.