Tissue plasminogen activator inhibits P-glycoprotein activity in brain endothelial cells

Authors
Deli, MA Abraham, CS Takahata, H Niwa, M
Citation
Ma. Deli et al., Tissue plasminogen activator inhibits P-glycoprotein activity in brain endothelial cells, EUR J PHARM, 411(1-2), 2001, pp. R3-R5
Citations number
8
Categorie Soggetti
Pharmacology & Toxicology
Journal title
EUROPEAN JOURNAL OF PHARMACOLOGY
ISSN journal
00142999 → ACNP
Volume
411
Issue
1-2
Year of publication
2001
Pages
R3 - R5
Database
ISI
SICI code
0014-2999(20010105)411:1-2<R3:TPAIPA>2.0.ZU;2-D
Abstract
Tissue plasminogen activator (0.01-30 mug/ml) dose-dependently inhibited th e functional activity of P-glycoprotein, assessed by rhodamine 123 accumula tion in GP8 immortalized rat brain endothelial cells, but this effect was u nrelated to its proteolytic activity. Elevation of intra-endothelial cyclic AMP concentration and stimulation of protein kinase C increased P-glycopro tein activity in GP8 cells and also attenuated the tissue plasminogen activ ator-induced inhibition. (C) 2001 Elsevier Science B.V. All rights reserved .