Birth of two infants who were seronegative for human immunodeficiency virus type 1 (HIV-1) after intracytoplasmic injection of sperm from HIV-1-seropositive men
D. Loutradis et al., Birth of two infants who were seronegative for human immunodeficiency virus type 1 (HIV-1) after intracytoplasmic injection of sperm from HIV-1-seropositive men, FERT STERIL, 75(1), 2001, pp. 210-212
Objective: To report two cases of live births after intracytoplasmic sperm
injection (ICSI) in two women who were seronegative for human immunodeficie
ncy virus type 1 (HIV-1) after the use of processed semen from their seropo
sitive husbands.
Design: Case reports.
Setting: University hospital IVF center.
Patient(s): Two HIV-1 seropositive men and their HIV-1 seronegative female
partners; all gave their informed consent in writing before undergoing the
ICSI procedures.
Intervention(s): The men provided semen samples that were processed with th
e use of Percoll and swim up techniques. Ovarian stimulation in the women w
as performed with the long protocol using GnRH analogs and recombinant FSH.
ICSI was performed.
Main Outcome Measure(s): Oocytes were fertilized by ICSI, and the resulting
embryos were transferred to the patients. The mothers and babies were test
ed for HIV-1 antibodies. Result(s): In the first case, seven mature oocytes
were collected and fertilized with ICSI, and three embryos were transferre
d; the woman became pregnant and gave birth to a healthy boy. Six months af
ter the birth, testing for HIV-1 antibodies in the woman and the baby gave
negative results. In the second case, 10 mature oocytes were collected and
fertilized with ICSI, and four embryos were transferred; the second woman b
ecame pregnant and also gave birth to a healthy boy. Testing for HIV-1 anti
bodies at the baby's delivery also gave negative results.
Conclusion(s): In women who are infertile because of fallopian tube obstruc
tion or in men who have poor quality semen for artificial insemination, ICS
I can be performed using processed semen. This method, which involves the u
se of only one spermatozoon per oocyte, provides HIV-1 seropositive men wit
h the opportunity to have children with a minimal risk-if any-of infecting
their female partners. (C) 2001 by American Society for Reproductive Medici
ne.