Beef cows (n = 473) from two locations were stratified by breed, postpartum
interval, age, and AI sire and were randomly allotted to one of four treat
ments for synchronization of ovulation. Ovulation synchronization protocols
included the Ovsynch protocol with (n = 114) or without (n = 123) 48-h cal
f removal from d 7 to 9 (d 0 = 1st GnRH injection) or the CO-Synch protocol
with (n = 119) or without (n = 117) 48-h calf removal from d 7 to 9. The O
vsynch protocol included administration of GnRH (100 mug; i.m.) on d O, PCF
2 alpha (25 mg; i.m.) on d 7, GnRH (100 mug; i.m.) on d 9, and timed insemi
nation on d 10. The GO-Synch protocol included administration of GnRH (100
mug; i.m.) on d O, PGF(2 alpha) (25 mg; i.m.) on d 7, and GnRH (100 mug; i.
m.) with timed insemination on d 9. Blood samples were collected from all c
ows on d -10 and d O for analysis of serum progesterone. Cows with at least
one serum progesterone concentration greater than 1 ng/mL were considered
to be cyclic at the time of treatment. Conception rates of cows that receiv
ed the GO-Synch + calf removal, Ovsynch + calf removal, GO-Synch, or Ovsync
h protocol (63, 61, 54, and 52%, respectively) were not different (P = 0.50
). Conception rates were not different (P = 0.80) among CO-Synch- and Ovsyn
ch-treated cows; however, both estrual status and 48-h calf removal affecte
d conception rates. Conception rates of cyclic cows (66%) were greater (P =
0.01) than those of anestrous cows (53%), regardless of which synchronizat
ion protocol was used. When data were pooled across synchronization protoco
l, conception rates of cows with 48-h calf removal (62%) were greater (P =
0.09) than conception rates of cows without calf removal (53%). The GO-Sync
h + calf removal protocol induces a fertile ovulation in cyclic and anestro
us cows, requires handling cattle just three times, results in high concept
ion rates from timed insemination, and should be a useful program for synch
ronization of ovulation in beef cows.