Pharmacological evidence for the selectivity of in vivo signals obtained with enzyme-based electrochemical sensors

Carbon fiber microelectrodes that support enzyme-containing redox polymer g els permit the amperometric detection of glutamate, choline, and glucose. T hese devices are of interest for in vivo neurochemical monitoring because t heir small dimensions may permit highly localized measurements within small brain nuclei. In vitro calibration procedures confirm that the sensors res pond in a selective fashion towards their respective target analyte. In the current work, the selectivity of the in vivo response of the microsensors during pharmacological manipulations is considered. The response of choline and glucose microsensors during the local infusion of tetrodotoxin and neo stigmine in rat striatum is reported. The results of this study support the conclusion that these microsensors respond selectively to their respective targets under in vivo conditions. (C) 2001 Elsevier Science B.V. All right s reserved.