RELATIONSHIP BETWEEN HUMAN GENOTYPE AND PHENOTYPE OF N-ACETYLTRANSFERASE (NAT2) AS ESTIMATED BY DISCRIMINANT-ANALYSIS AND MULTIPLE LINEAR-REGRESSION .1. GENOTYPE AND N-ACETYLATION IN-VIVO

Twenty-six healthy Caucasian subjects were evaluated for polymorphic N -acetyltransferase (NAT2) metabolic activity in vivo bg sulfamethazine phenotyping and for their respective NAT2 genotype. Application of di scriminant analysis allowed the separation of the rapid and slow acety lators solely on the base of their respective mutation pattern with id entical results as a achieved by the classical method of discriminatio n according to the phenotyping results. Multiple linear regression ana lysis was used to obtain a quantitative relationship between allelic p attern and the phenotypic outcome, It is shown that the computation me thods produce relationships enabling the influence of particular mutat ions and/or allelic configurations on the metabolic activity in vivo t o be estimated, This may be important in cases of discordant or overla pping phenotype and genotype results as well as in investigating the N AT2 polymorphism as a risk factor for cancer and other disease in epid emiological studies.