alpha-chymotrypsin-catalysed peptide synthesis via the kinetically controlled approach using activated esters as acyl donors in organic solvents withlow water content: incorporation of non-protein amino acids into peptides

The coupling efficiency in the alpha -chymotrypsin-catalysed peptide synthe sis via the kinetically controlled approach is greatly improved by the use of activated eaters such as the 2,2,2-triAuoroethyl ester as acyl donors in stead of the conventional methyl ester in organic solvents such as acetonit rile with low water content. This approach is useful for the incorporation of non-protein amino acids such as halogenophenylalanines into peptides.