Immunochemical assessment of neural visinin-like calcium-binding protein 3expression in rat brain

Citation
H. Hamashima et al., Immunochemical assessment of neural visinin-like calcium-binding protein 3expression in rat brain, NEUROSCI RE, 39(1), 2001, pp. 133-143
Citations number
46
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROSCIENCE RESEARCH
ISSN journal
01680102 → ACNP
Volume
39
Issue
1
Year of publication
2001
Pages
133 - 143
Database
ISI
SICI code
0168-0102(200101)39:1<133:IAONVC>2.0.ZU;2-3
Abstract
Expression of neural visinin-like calcium-binding protein 3 (NVP3) was asse ssed by immunoblot and immunohistochemical analyses in rat brain. NVP3 was markedly expressed in the cerebellum, at a concentration of 9.5 muM. On SDS -PAGE, native NVP3 migrated at 23kDa, identical to the recombinant myristoy l-form, but somewhat faster than the non-myristoyl-form. Both forms bound 3 moles of calcium. The myristoyl-form exhibited a cooperativity in binding calcium and calcium-dependent membrane-binding. but the non-myristoyl-form did not. At 3 months. NVP3 was primarily localized in the Purkinje cells, w ith intense staining in the cell bodies, dendrites and axons. The cerebella r granule cells and basal nuclear neurons were faintly stained. During deve lopment of the cerebellum NVP3-positive Purkinje cells first appeared on po st-natal day 14 (P14). The staining intensity then increased and plateaued on P28. Labeling showed a tendency to accumulate in the dendrites and nerve terminals in a fine granular pattern. During aging process. NVP3 levels de creased by 43% at 12 months and 68% at 24 months, while the levels of NVP1, synaptophysin and drebrin were preferentially preserved. These results sug gest that NVP1 is involved in dendritic arborization and postsynaptic funct ion in cerebellar Purkinje cells and that presynaptic nerve terminals are a nother functional site of the protein. (C) 2001 Elsevier Science Ireland Lt d and the Japan Neuroscience Society. All rights reserved.