Citation
Bl. Zhang et al., Synthesis of 5 '-deoxy-5 '-thioguanosine-5 '-monophosphorothioate and its incorporation into RNA 5 '-termini, ORG LETT, 3(2), 2001, pp. 275-278
Citations number
34
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
ORGANIC LETTERS
ISSN journal
15237060
→ ACNP
Volume
3
Issue
2
Year of publication
2001
Pages
275 - 278
Database
ISI
SICI code
1523-7060(20010125)3:2<275:SO5'''>2.0.ZU;2-S
Abstract
[GRAPHICS]
5'-Deoxy-5'-thioguanosine 5' monophosphorothioate (GSMP) was synthesized in
four steps with 35% overall yield, GSMP serves as a good substrate for in
vitro transcription with T7 RNA polymerase to yield 5'-GSMP-RNA, which was
converted to 5'-HS RNA by dephosphorylation with alkaline phosphatase. The
thiol-reactive agents can be efficiently introduced into the 5'-terminus of
RNA.