Zinc bioavailability in tetrabasic zinc chloride and the dietary zinc requirement of young chicks fed a soy concentrate diet

Three chick assays were conducted to determine a bioavailable Zn requiremen t and to establish a Zn relative bioavailability (RBV) value for tetrabasic Zn chloride [TBZC, i.e., Zn5Cl2(OH)(8)]. In Assay 1, 8-d-old chicks were f ed a Zn-deficient soy concentrate diet (8.8 mg/kg bioavailable Zn) suppleme nted with 0, 5.81, 10.81, 15.10, and 20.25 mg Zn/kg from ZnSO4. 7H(2)O for 14 d to establish a linear response range and estimate a bioavailable Zn re quirement. Weight gain of chicks increased linearly (P < 0.01; r(2) = 0.99) when the first three levels of Zn were fed. Broken-line analysis of the we ight gain data indicated a bioavailable Zn requirement of 22.4 mg/kg. In As say 2, RBV of Zn in TBZC was determined by multiple-linear regression slope -ratio methodology. The Zn-deficient basal diet was supplemented with 0, 5. 81, and 10.81 mg Zn/kg from ZnSO4<bullet>7H(2)O to produce a linear growth response as determined in Assay 1. The same basal diet was supplemented wit h 5.38 and 10.81 mg Zn/kg from TBZC. The RBV of Zn in TBZC was found to be 102%, which was not different (P > 0.10) from 100%. A third assay was condu cted as described for Assay 2, and the RBV of Zn in TBZC was 111%, which wa s not different from 100%. In summary, the RBV of Zn in TBZC was not differ ent from that of ZnSO4.H2O, and the minimal bioavailable Zn requirement for chicks 1 to 3 wk of age fed a soy concentrate diet was estimated at 22.4 m g/kg.