Anion binding to porcine pancreatic alpha-amylase as probed by bromine-81 nuclear magnetic resonance spectrometry

Authors
Yoshimura, E Tachibe, M Mori, S Yamazaki, S
Citation
E. Yoshimura et al., Anion binding to porcine pancreatic alpha-amylase as probed by bromine-81 nuclear magnetic resonance spectrometry, SPECT LETT, 33(6), 2000, pp. 893-899
Citations number
10
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
SPECTROSCOPY LETTERS
ISSN journal
00387010 → ACNP
Volume
33
Issue
6
Year of publication
2000
Pages
893 - 899
Database
ISI
SICI code
0038-7010(2000)33:6<893:ABTPPA>2.0.ZU;2-D
Abstract
A bromine-81 NMR study was conducted to characterize the Cl--binding site o f porcine pancreatic alpha -amylase. Only a single signal was observed in t he spectrum in the presence of an equimolar concentration of the enzyme and NaBr. The signal was assigned to free Br- ions, which are in very slow exc hange with the protein-bound Br- on an NMR time scale. The presence of a co mmon anion-binding site was demonstrated from the competition of Br- with F -,Cl-, NO3-, and ClO4- for the site.