Bt. Scott et al., Genetic screening of candidate genes for a prothrombotic interaction with type I protein C deficiency in a large kindred, THROMB HAEM, 85(1), 2001, pp. 82-87
The incomplete penetrance of thrombosis in familial protein C deficiency su
ggests disease occurs when this deficit is combined with additional abnorma
lities in the hemostatic system. The pattern of inherited thrombophilia in
the Vermont II kindred, which is affected by a clinically dominant type I p
rotein C deficiency, provides strong evidence for a second unidentified gen
e that segregates independently of protein C deficiency and increases susce
ptibility to thrombosis. To test the second gene hypothesis, thirty-four ca
ndidate genes for proteins involved in hemostasis or inflammation were test
ed as the unknown defect, using highly polymorphic short tandem repeat (STR
) markers in an informative subset (n = 31) of the kindred. The genes consi
dered are; alpha -fibrinogen, beta -fibrinogen, gamma -fibrinogen, prothrom
bin, tissue factor, factor V, protein S, complement component 4 binding pro
tein, factor XI, factor XII, factor XIIIa, factor XIIIb, histidine rich gly
coprotein, high molecular weight kininogen, kallikrein, von Willebrands fac
tor, platelet factor 4, thrombospondin, antithrombin III, alpha -1-antitryp
sin, thrombomodulin, plasminogen, tissue plasminogen activator, urokinase p
lasminogen activator, plasminogen activator inhibitor-1, plasminogen activa
tor inhibitor-2, protein C inhibitor, alpha -2-plasmin inhibitor, kallistat
in, lipoprotein a, interleukin 6, interleukin 1,cystathionine-beta -synthas
e, and methylenetetrahydrofolale reductase. Mutations in many of these gene
s have been previously established as independent risk factors for thrombos
is. However, linkage analysis provided no evidence to implicate any of the
candidate genes as the second inherited factor that promotes thrombophilia
in this kindred.