A time-resolved fluoroimmunometric assay for the detection of microcystins, cyanobacterial peptide hepatotoxins

Authors
Mehto, P Ankelo, M Hinkkanen, A Mikhailov, A Eriksson, JE Spoof, L Meriluoto, J
Citation
P. Mehto et al., A time-resolved fluoroimmunometric assay for the detection of microcystins, cyanobacterial peptide hepatotoxins, TOXICON, 39(6), 2001, pp. 831-836
Citations number
23
Categorie Soggetti
Pharmacology & Toxicology
Journal title
TOXICON
ISSN journal
00410101 → ACNP
Volume
39
Issue
6
Year of publication
2001
Pages
831 - 836
Database
ISI
SICI code
0041-0101(200106)39:6<831:ATFAFT>2.0.ZU;2-6
Abstract
An immunoassay based on the time-resolved fluorometry (TR-FIA) was develope d for microcystins, cyanobacterial peptide hepatotoxins. The assay was perf ormed in a competitive mode and it utilised the monoclonal antibodies raise d against microcystin-LR, and a europium chelate of microcystin-LR as a com petitive antigen. The sensitivity of the assay was 0.1 mug/l. The derection method of TR-FIA was compared to a commercially available kit based on the enzyme-linked immunosorbent assay (ELISA). The same level of sensitivity c ould be obtained with TR-FIA (in a non-optimised system). The simplified me thod of TR-FIA leads to a shorter analysis time. (C) 2001 Elsevier Science Ltd. All rights reserved.