Functional characteristics of S-59 photochemically treated platelet concentrates derived from buffy coats

Background: A photochemical treatment (PCT) process for inactivation of inf ectious pathogens and leukocytes has been developed and evaluated using sin gle-donor platelet concentrates. This study assessed the application of PCT to platelets prepared from pooled buffy coats. In this study, in vitro fun ctional characteristics of PCT platelets were compared to control platelets prepared from pooled buffy coats using the approved platelet-additive solu tion T-Sol(R). Platelets in platelet PAS III additive solution without PCT were evaluated as well. PCT also included the use of a psoralen (S-59) redu ction device (SRD). Materials and Methods: Four types of platelet concentra tes were compared: (I)platelet concentrate in plasma/T-Sol; (2) platelet co ncentrate in plasma/PAS III; (3) platelet concentrate in plasma/PAS III, PC T, 9 h SRD and (4) platelet concentrate in plasma/PAS III, PCT, 16 h SRD. P CT occurred on the day after whole-blood collection. In vitro assay paramet ers included: pH, PO2, pCO(2), HCO3-, platelet count, mean platelet volume, plasma glucose, plasma lactate, total ATP, expression of p-selectin, hypot onic shock response and electron microscopy. Results: The results indicate that PCT is compatible with platelet concentrates prepared from pooled buff y coats for up to 7 days of storage. Conclusion: The PCT process resulted i n acceptable in vitro platelet functional characteristics and is currently in clinical trials to evaluate the haemostatic efficacy of PCT platelets in thrombocytopenic patients requiring multiple platelet transfusions. Copyri ght (C) 2000 S. Karger AG, Basel.