Induction of polyploidization by jaspamide in HL-60 cells

Jaspamide, a natural peptide isolated from the marine sponge Hemiastrella m inor, was used in the study of polyploidy in HL-60 cells, Jaspamide at 5 x 10(-8) M concentration exhibited antiproliferative activity and an increase d CD4 and CD14 surface expression, After 2 days of cultivation, 56.3% of th e exposed cells became multinuclear compared with 2.4% in controls. Moreove r, the size and the number of nuclei of the cells increased in a time-depen dent manner, An increased number of meta phase chromosomes was observed by microscopical enumeration after colcemid treatment for 60 min. The analysis of the DNA content of these cells, measured by propidium iodide staining, revealed a significant increase in the cells percentage with increased DNA content, Taken together, these findings indicate that the jaspamide treatme nt induces polyploidization in the HL-60 cell line, Copyright (C) 2001 S. K arger AG, Basel.