PROBLEM: Neither the integrin pattern nor the biological functions of integ
rins have been extensively documented in human cultured testicular peritubu
lar myoid cells (TPMC). The integrin pattern and the presence of some prote
ins of the immunoglobulin superfamily on human TPMC as well as the role of
integrins in TPMC contraction were examined.
METHOD OF STUDY: Integrin expression was evaluated bq immunofluorescence an
d FACS analysis. To assess the role of integrin in TPMC contraction, human
and rat cells were added to a collagen gel system and exposed to contractil
e stimuli.
RESULTS: The immunofluorescence and cytofluorimetric analysis showed that h
uman cultured TPMC express alpha (1), alpha (2), alpha (3), alpha (5), alph
a (6), alpha (v), beta (1), beta (3), and beta (4) integrin subunits, and s
ignificant amounts of intercellular adhesion molecule-1 (ICAM-1), whereas t
hey do not present alpha (4), beta (2), beta (7) subunits, nor intercellula
r adhesion molecule-2 (ICAM-2) and neural cell adhesion molecule (NCAM). Th
e preincubation of human cells with an anti-beta (1) mAb and of rat cells w
ith a polyclonal anti-beta (1) antibody inhibited TPMC contraction induced
by different contractile stimuli.
CONCLUSION: Our investigation documented a broad integrin pattern on human
cultured TPMC as well as a role for integrins in human and rat TPMC contrac
tion.