PentaLyte (R) does not decrease heparinoid release but does decrease circulating thrombotic mediator activity associated with aortic occlusion-reperfusion in rabbits

Hemorrhage and thrombosis are associated with major vascular and trauma sur gery. Release of heparinoids and thrombotic mediators may contribute to the se complications and have been described in rabbits after aor tic occlusion -reperfusion. We hypothesized that the resuscitative fluid used could reduc e heparinoid and thrombotic mediator release after aortic occlusion-reperfu sion in rabbits as assessed by thromboelastographic variables (R, reaction time; alpha, angle; and G, a measure of clot strength). Anesthetized rabbit s were administered lactated Ringer's solution (n = 8) or Pentalyte(R) (n = 8) at reperfusion after 30 min of ischemia. Blood was obtained before isch emia and after 30 min of reperfusion for thromboelastography under four con ditions: 1) unmodified sample, 2) platelet inhibition, 3) heparinase, and 4 ) platelet inhibition and heparinase. During reperfusion, unmodified sample s demonstrated a significant increase in A and decrease in or and G that wa s not affected by PentaLyte(R). In the presence of heparinase, no significa nt fluid-specific thromboelastographic differences were noted. However, thr ombotic mediator release (discerned by a decrease in R and an increase in o r) during reperfusion in samples with platelet inhibition and heparinase wa s significantly attenuated by PentaLyte(R). PentaLyte(R) administration doe s not decrease heparinoid release but does decrease thrombotic mediator rel ease after aortic occlusion-reperfusion.