Different transendothelial migration behaviour pattern of blood monocytes derived from patients with benign and malignant diseases of the breast

Background: In this study we compared the expression of selected monocyte s urface antigens with the potential to transmigrate through an endothelial l ayer before and after surgery from breast cancer patients (CA) and patients with benign disease of the breast (BE). Materials and Methods: Transmigrat ion capacity of mononuclear cells was determined after isolation by Ficoll density gradient, layered over human umbilical vein endothelial cells and c ultured in a two chamber plate added with fMLP as a chemotactic stimulus. W e determined monocyte phenotye (HLA-DR, FcgRI/CD64, CR1/CD11b and LFA-1/CD1 1a) and the phagocytosis off. coli by flow cytometry. Results: Before surge ry blood monocytes had an equal expression of the measured surface antigens , but were different in regard to their interaction with endothelial cells. Monocytes derived from CA had a higher a transmigration potency than those of BE. Moreover; the migration through the endothelial cell layer created different populations of monocytes, Surgical stress modified transmigrated monocytes of BE into the direction of monocytes from CA. Phagocytic capacit y of peripheral blood monocytes fr om CA was significantly diminished and w as further reduced after surgery when measured in transmigrated cells Concl usion, Our study shows that monocytes from CA and BE can be discriminated i n regard to their interaction with endothelial cells.