Synergistic effect of the angiogenesis inhibitor TNP-470 and tumor necrosis factor (TNF) on Bomirski Ab melanoma in hamsters

Bomirski Ab transplantable melanoma bearing hamsters were treated with angi ogenesis inhibitor TNP-470 or with rat tumor necrosis factor (TNF) at close s of 20 mug and 40 mug or with both TNP-470 and TNF at a dose of 20 mug eac h. The size of the growing tumor was measured everyday from the day when it was palpable. After the death of the animals the final size of their tumor s was measured and the number and localisation of metastasis were determine d. It was found that the rate of tumor growth was lowest in the group of an imals treated with TNF at a dose of 40 mug and in the group of animals trea ted with TNP-470 and TNF at a dose of 20 mug. The frequency of metastasis w as different in the experimental groups although their location was similar . The longest living animals belonged to the group treated with both TNP-47 0 and TNF. The results of the investigation indicate that TNP-470 and TNF a ct in a synergistic way that allows a nea ease in the effective dose of TNF thus diminishing its noxious side effects.