SELF-ASSEMBLING OF GLUTATHIONE S-TRANSFERASE CALMODULIN FUSION PROTEIN ON CHEMICALLY-MODIFIED GOLD SURFACE/

The fusion protein technique was used to prepare an artificial polyfun ctional protein from calmodulin (CaM) and glutathione S-transferase (G ST). The fusion protein was designed, expressed, purified, and then as sembled to the glutathione self-assembled gold surface. The protein as sembly was confirmed through enzyme binding assay and enzyme immunoass ay. Specific binding of the fusion protein to glutathione self-assembl ed on the gold surface was assessed via a quartz crystal microbalance (QCM). The fusion protein was reversibly adsorbed and desorbed by the competitive binding of glutathione present in a solution, thus showing that the binding of the fusion protein was specific and had a highly oriented molecular configuration. (C) 1997 Elsevier Science B.V.