AN IN-VITRO PHARMACOLOGICAL EXAMINATION OF VENOM FROM THE SOLDIERFISHGYMNAPISTES MARMORATUS

The aim of the present study was to commence a characterisation of som e of the basic pharmacological properties of venom from the soldierfis h (Gymnapistes marmoratus). Soldierfish venom was prepared by extracti on into 10% glycerol and centrifugation to remove insoluble material. Protein content was determined and venom concentrations were expressed as mu g venom protein. Soldierfish venom (0.5-15 mu g/ml) produced co ncentration-dependent contractile responses in guinea-pig isolated ile um (GPI) and longitudinal smooth muscle (LSM) preparations. The muscar inic receptor antagonist atropine (10 nM) significantly inhibited resp onses of LSM to soldierfish Venom (2.5 mu g/ml). Responses to soldierf ish venom (4-5 mu g/ml) in GPI were not significantly affected by the ganglion-blocking drug mecamylamine (10 mu M) or by incubation with bl ood cholinesterase, The cyclooxygenase inhibitor indomethacin (2 mu M) significantly inhibited responses to soldierfish venom (2.5 mu g/ml) in LSM. Neither the thromboxane A(2)/prostaglandin H-2 receptor antago nist GR32191B (1 mu M) nor the leukotriene receptor antagonist SB20531 2 (10 nM) significantly affected responses to soldierfish venom (5 mu g/ml) in GPI. Responses to soldierfish venom (2.5-5 mu g/ml) were not significantly inhibited by the histamine receptor antagonist mepyramin e (0.5 mu M), the angiotensin-converting enzyme inhibitor captopril (2 mu M) or the neurokinin-1 receptor antagonist CP-99,994 (0.1 mu M) in LSM. The angiotensin AT, receptor antagonist EXP3174 (0.1 mu M) also failed to inhibit significantly the responses to soldierfish venom (5 mu g/ml) in GPI, A fluorometric assay for the detection of 5-hydroxytr yptamine (5-HT) and related compounds indicated a level in soldierfish venom of 1.60 +/- 0.01 ng of 5-HT-like substance per mu g venom prote in. Soldierfish venom (0.5-10 mu g/ml) produced concentration-dependen t contractile responses in rat isolated stomach fundus strips, and the se responses (2.5 mu g/ml) were significantly inhibited by the 5-HT1/5 -HT2 receptor antagonist methysergide (0.1 mu M). These results sugges t that soldierfish venom may stimulate the release of acetylcholine to act at muscarinic receptors on guinea-pig gastrointestinal smooth mus cle, The venom also appears to be causing the release of cyclooxygenas e products, such as prostaglandins, and contains 5-HT, or a 5-HT-like substance, that acts directly at 5-HT receptors. (C) 1997 Elsevier Sci ence Ltd.